IN VITRO EVALUATION OF A PILE LOOP KNITTED SCAFFOLD FOR MYOCARDIAL TISSUE ENGINEERING USING C2C12 CELLS

Pile loop knitted scaffold has attracted attention for being used as a three dimensional (3D) biocompatible material particularly the application of myocardial tissue engineering. In this research, nonintermingled, textured polyethylene terephthalate (PET) multifilament yarn with filament count 48 was producing pile knit structure to investigate if could provide an appropriate microenvironment seeded murine C2C12 myoblasts in case increasing diameter (20.7 micron versus 9.1 from previous study). The resulting had pore network mainly consisting macropores 100-200 µm that were well interconnected micropores (<90 µm). Furthermore, mean values Young’s modulus structures at 20% strain 240.67 kPa warp direction, and 85.73 weft which can be claimed not far those declared literature human heart muscle. addition, cyclic loading-unloading test revealed no creep fabric after 100 cycles. According results WST-1 assay, filament-based non-cytotoxic cells able proliferate properly on scaffold, visualized by SEM confocal images. respect, keep promising cardiac patch.